Synonyms
RAD2
Uniprot ID
P39748
Form
liquid
French translation
anticorps
Specificity
Human, Mouse
Tested Application
ELISA, WB, IHC
Calculated MW
Refer to Figures
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Immunogen
flap structure-specific endonuclease 1
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:10 - 1:100; IP 1:50 - 1:200
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab03075(FEN1 antibody) at dilution of 1:1000
Image1
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab03075( FEN1 Antibody) at dilution of 1:50
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
