Uniprot ID
P16104
Calculated MW
16 kDa
Form
liquid
French translation
anticorps
Specificity
Human, Mouse, Rat
Tested Application
ELISA, WB, IHC, IF
Purification
Immunogen affinity purified
Immunogen
H2A histone family, member X
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:100 - 1:200; IF: 1:50 - 1:200
Synonyms
H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image1
Immunohistochemistry of paraffin-embedded human breast cancer using FNab03885(H2AFX antibody) at dilution of 1:100
Image4
HL-60 cells were subjected to SDS PAGE followed by western blot with FNab03885(H2AFX antibody) at dilution of 1:1000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
