Uniprot ID
O60921
Form
liquid
French translation
anticorps
Tested Application
ELISA, IHC, WB
Calculated MW
Refer to figures
Specificity
Human, Mouse, Rat
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Immunogen
HUS1 checkpoint homolog (S. pombe)
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Image1
Immunohistochemistry of paraffin-embedded human colon cancer using FNab04082(HUS1 antibody) at dilution of 1:50
Image4
BxPC-3 cells were subjected to SDS PAGE followed by western blot with FNab04082(HUS1 antibody) at dilution of 1:0
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.