Uniprot ID
P02686
Calculated MW
34 kDa
Form
liquid
French translation
anticorps
Specificity
Human, Mouse, Rat
Tested Application
ELISA, WB, IHC, IF
Immunogen
myelin basic protein
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Synonyms
MBP, Myelin A1 protein, Myelin basic protein
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image1
Immunohistochemistry of paraffin-embedded human brain tissue slide using FNab05465( MBP Antibody) at dilution of 1:200
Image4
mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab05465( MBP Antibody) at dilution of 1:10000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
Background
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
