Uniprot ID
P07196
Calculated MW
82 kDa
Form
liquid
French translation
anticorps
Specificity
Human, Mouse, Rat
Tested Application
ELISA, WB, IHC, IF
Purification
Immunogen affinity purified
Immunogen
neurofilament, light polypeptide
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Synonyms
68 kDa neurofilament protein, CMT1F, CMT2E, FLJ53642, NEFL, NF L, NF68, NFL
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image1
Immunohistochemistry of paraffin-embedded human brain tissue slide using FNab05710( NEFL Antibody) at dilution of 1:200
Image4
mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab05710(NEFL antibody) at dilution of 1:10000
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.