anti- PSMC2 antibody

Size

2X100μg

Catalog no#

FNab06878

Price

320 EUR

Synonyms

MSS1

Uniprot ID

P35998

Form

liquid

Calculated MW

125 kDa

French translation

anticorps

Specificity

Human, Mouse

Tested Application

ELISA, WB, IHC, IF

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Immunogen

proteasome (prosome, macropain) 26S subunit, ATPase, 2

Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200

Image1

Immunohistochemistry of paraffin-embedded human ovary tumor using FNab06878(PSMC2 antibody) at dilution of 1:100

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Image4

human adrenal gland tissue were subjected to SDS PAGE followed by western blot with FNab06878(PSMC2 antibody) at dilution of 1:1000

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms.