Uniprot ID
P61586
Form
liquid
French translation
anticorps
Tested Application
ELISA, WB, IHC
Calculated MW
Refer to figures
Specificity
Human, Mouse, Rat
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Immunogen
ras homolog gene family, member A
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Synonyms
ARH12, ARHA, h12, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
HeLa cells were subjected to SDS PAGE followed by western blot with FNab07281(RHOA antibody) at dilution of 1:1000
Image1
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab07281( RHOA Antibody) at dilution of 1:200
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.