Uniprot ID
P84022
Calculated MW
48 kDa
Form
liquid
French translation
anticorps
Tested Application
ELISA, WB, IHC
Specificity
Human, Mouse, Rat
Immunogen
SMAD family member 3
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Image4
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab07994( SMAD3 Antibody) at dilution of 1:1000
Image1
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab07994( SMAD3 Antibody) at dilution of 1:50
Properties
If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Synonyms
MADH3, hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
Background
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.