anti- Phospho-CHEK1-S280 antibody

Size

2X100μg

Catalog no#

FNab09816

Price

320 EUR

Synonyms

CHK1

Uniprot ID

O14757

Calculated MW

60 kDa

Form

liquid

French translation

anticorps

Specificity

Human, Mouse

Tested Application

ELISA, WB, IHC, IF

Immunogen

Phospho-CHEK1-S280

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200

Image1

Immunofluorescence analysis of MCF-7 cells using FNab09816. Blue: DAPI for nuclear staining.

Image4

HT-29 cells were subjected to SDS PAGE followed by western blot with FNab09816 at dilution of 1:1000

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.