Rabbit Anti- CD9 Polyclonal: HRP

Size

100 µl

Catalog no#

SPC-1272D-HRP

Price

344 EUR

Antibody's target

CD9

Gene number

928

Total weight (kg)

1.4

Net weight (g)

0.1

Country of production

Canada

Protein number

P21926

Storage recommendations

-20°C

Raised in

Rabbit

Antibody in cell

Membrane

Stock availabilit

In Stock

Released date

6/Sep/2016

NCBI number

NP_001760.1

Tested reactivity

To be tested

Tested applications

To be tested

Warning information

Non-hazardous

Conjugation

HRP Conjugated

PubMed number

Refer to PubMed

Verified applications

WB, IHC, ICC/IF

Shipping recommendations

Blue Ice or 4°C

Representative figure link

No Data Available

Antibody's reactivity

Human, Mouse, Rat

Antibody's category

Polyclonal Antibodies

Latin name

Oryctolagus cuniculus

Immunogen

A synthesized peptide

Antibody's purified from

Peptide Affinity Purified

Group

Polyclonals and antibodies

Antibody's full description

Rabbit Anti- CD9 Polyclonal

Antibody's specificity

Detects endogenous levels of total CD9

Antibody's concentration

See included datasheet or contact our support service

Recommended buffer for storage

PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide

Tissue specificity

Expressed by a variety of hematopoietic and epithelial cells.

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Primary research fields

Immunology, Cell Type Markers, CD Markers, Stem Cells, Embryonic Stem Cells, ESC Surface Molecules

Antibody's recommended dilutions for use

WB (1:1000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Certificate of analysis

A 1:1000 dilution of SPC-1272 was sufficient for detection of CD9 in 10 µg of COLO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Bibliography

1. Miao W.M., et al. (2001) Blood. 97:1689. 2. Ellerman D.A., et al. (2003) Mol. Biol Cell. 14: 5098-5103. 3. Schlossman S., et al. (1995) Leucocyte Typing V. Oxford University Press. New York.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Properties

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Antibody's other name

CD9 antigen Antibody, CD9 antigen p24 Antibody, Leukocyte antigen MIC3 Antibody, Motility-related protein Antibody, Tetraspanin-29 Antibody, Tspan-29 Antibody, TSPAN29 Antibody, BA-2/p24 antigen Antibody, BA2 Antibody, Cell growth inhibiting gene 2 protein Antibody, DRAP 27 Antibody, GIG2 Antibody, Growth inhibiting gene 2 protein Antibody

Scientific context

CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β1 integrins. It plays a role in cell signaling by mediating interactions with low molecular weight GTP-binding proteins.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.