Mouse Anti- Nitrotyrosine Monoclonal IgG2a, Clone 39B6

Size

100 µg

Catalog no#

SMC-154D-P594

Price

415 EUR

Net weight (g)

0.1

Total weight (kg)

1.4

Clone

39B6

Raised in

Mouse

Additional isotype

IgG2a

Storage recommendations

-20ºC

Country of production

Canada

Antibody's concentration

1 mg/ml

Stock availability

In Stock

Released date

1-Nov-2007

Latin name

Mus musculus

Antibody's target

Nitrotyrosine

NCBI number

Refer to NCBI

Warning information

Non-hazardous

Shipping recommendations

Blue Ice or 4ºC

Gene number

Refer to GenBank

Purity

Protein G Purified

Protein number

Refer to Swiss-Prot

Antibody's reactivity

Species Independent

Immunogen

Hybridoma line 39B6

Brandname

MOLECULAR SIGNATURE®

Antibody's category

Monoclonal Antibodies

Antibody's datasheet

Contact our support service

Antibodies' applications

WB, IHC, ICC/IF, IP, ELISA, FCM, AM

Antibody buffer for storage

PBS, 50% glycerol, 0.09% sodium azide

Antibody's other name

Nitro tyrosine Antibody, 3-Nitrotyrosine Antibody

Tissue specificity

See included datasheet or contact our support service

Antibody in cell

See included datasheet or contact our support service

Antibody's full description

Mouse Anti- Nitrotyrosine Monoclonal IgG2a Antibody, Clone: 39B6: PE/ATTO 594

Antibody's dilutions

WB (1:1400), IHC (1:100); optimal dilutions for assays should be determined by the user.

Tested reactivity

Mamalian, Cattle, goat, sheep, cat, dog, horse, Rat, Rat, Rat, Human, Human, Rat, Mouse, Mouse

PubMed number

27514800, 26239911, 25882840, 25864985, 25730373, 24909615, 24909615, 24679269, 23077100, 20129246

Antibody's specificity

Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Antibody certificate of analysis

0.7 µg/ml of SMC-154 was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Primary research fields

Cancer, Oxidative Stress, Cell Signaling, Post-translational Modifications, Nitration, Neuroscience, Neurodegeneration, Alzheimer's Disease, Parkinson's Disease

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Tested applications

Antibody Microarray, Flow Cytometry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Bibliography

1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387. 2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8. 3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30. 4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45. 5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199. 6. Khan J. et al. (1998) Biochem J. 330(2): 795-801.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Representative figure link

| | | |

Scientific context

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis (4).

Representative figure legend

Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (SMC-154). Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (SMC-154) at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute. Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (SMC-154). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (SMC-154) at 1:1000000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (SMC-154). Tissue: liver tissue . Species: Rat. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (SMC-154) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (SMC-154). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (SMC-154) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (SMC-154). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (SMC-154) at 1:25000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. Mouse Anti-Nitrotyrosine Antibody [39B6] used in Immunohistochemistry (IHC) on Mouse Retinal Injury Model (SMC-154) Mouse Anti-Nitrotyrosine Antibody [39B6] used in Immunohistochemistry (IHC) on Mouse inflamed colon (SMC-154) Mouse Anti-Nitrotyrosine Antibody [39B6] used in Immunohistochemistry (IHC) on Rat liver tissue (SMC-154) Mouse Anti-Nitrotyrosine Antibody [39B6] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-154) Mouse Anti-Nitrotyrosine Antibody [39B6] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-154)