Mouse Anti- Phosphotyrosine Monoclonal IgG1, Clone G104

Size

100 µg

Catalog no#

SMC-174D-ALP

Price

379 EUR

Net weight (g)

0.1

Total weight (kg)

1.4

Clone

G104

Brandname

None

Additional isotype

IgG1

Raised in

Mouse

Country of production

Canada

Storage recommendations

-20ºC

Antibody's concentration

1 mg/ml

Stock availability

In Stock

Released date

1-Jan-2010

Tested applications

To be tested

Tested reactivity

To be tested

Latin name

Mus musculus

NCBI number

Refer to NCBI

Warning information

Non-hazardous

Antibody's target

Phosphotyrosine

Shipping recommendations

Blue Ice or 4ºC

Gene number

Refer to GenBank

Purity

Protein G Purified

Antibodies' applications

WB, IHC, ICC/IF, IP

Protein number

Refer to Swiss-Prot

Antibody's reactivity

Species Independent

Antibody's category

Monoclonal Antibodies

Antibody's datasheet

Contact our support service

Antibody buffer for storage

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Tissue specificity

See included datasheet or contact our support service

Antibody in cell

See included datasheet or contact our support service

Antibody's other name

PhosphoTyrosine (pY) Antibody, PhosphoTyrosine (pY) Antibody

Primary research fields

Cell Signaling, Post-translational Modifications, Phosphorylation

Antibody's full description

Mouse Anti- Phosphotyrosine Monoclonal IgG1 Antibody, Clone: G104: Alkaline Phosphatase

Antibody's dilutions

WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Immunogen

Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide

Antibody's specificity

Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Antibody certificate of analysis

1 µg/ml of SMC-174 was sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Representative figure link

|

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Bibliography

1. Garton A.J., Flint A.J., and Tonks N.K. (1996) Mol and Cell Bio 16(11): 6408-6418. 2. Garton A.J., and Tonks N.K. (1999) J Bio Chem. 274(6): 3811-3818. 3. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 4. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 5. Downward J. (2001) Nature 411: 759-762. 6. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 7. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352. 8. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656. 9. Tiganis T., Kemp B.E., and Tonks N.K. (1999) J. Bio Chem. 274(39): 27768-27775.

Representative figure legend

Immunohistochemistry analysis using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone G104 (SMC-174). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Phosphotyrosine Monoclonal Antibody (SMC-174) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Stratum granulosum staining in the epidermis. Some dermal staining. Western Blot analysis of Human A431 cell lysates showing detection of Phosphotyrosine protein using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone G104 (SMC-174). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Phosphotyrosine Monoclonal Antibody (SMC-174) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Left: normal, right: EGF treated. Mouse Anti-Phosphotyrosine Antibody [G104] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-174) Mouse Anti-Phosphotyrosine Antibody [G104] used in Western Blot (WB) on Human A431 cell lysates (SMC-174)

Scientific context

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).