Mouse Anti- Alginate Monoclonal IgG1 Kappa, Clone 3G4-1F5

Size

100 µg

Catalog no#

SMC-209D-ALP

Price

411 EUR

Net weight (g)

0.1

Total weight (kg)

1.4

Additional isotype

IgG1

Brandname

None

Raised in

Mouse

Country of production

Canada

Storage recommendations

-20ºC

Clone

3G4-1F5

Antibodies' applications

WB, IHC

Antibody's concentration

1 mg/ml

Antibody's target

Alginate

Stock availability

In Stock

Released date

24-Jun-2014

Tested applications

To be tested

Tested reactivity

To be tested

Latin name

Mus musculus

Warning information

Non-hazardous

NCBI number

Refer to NCBI

Gene number

Refer to GenBank

Shipping recommendations

Blue Ice or 4ºC

Purity

Protein G Purified

Antibody's reactivity

Species Independent

Protein number

Refer to Swiss-Prot

Antibody's category

Monoclonal Antibodies

Antibody's datasheet

Contact our support service

Immunogen

Sodium Alginate conjugated to KLH

Antibody buffer for storage

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Primary research fields

Cell Signaling, Post-translational Modifications

Antibody in cell

See included datasheet or contact our support service

Tissue specificity

See included datasheet or contact our support service

Antibody's other name

Alginic Acid Antibody, Algin Antibody, Sodium Alginate Antibody

Antibody's dilutions

WB (1:500); optimal dilutions for assays should be determined by the user.

Antibody's specificity

Binds selectively to a BSA-conjugated alginate, but not to unconjugated BSA.

Antibody's full description

Mouse Anti- Alginate Monoclonal IgG1 Kappa Antibody, Clone: 3G4-1F5: Alkaline Phosphatase

Antibody certificate of analysis

1 µg/ml of SMC-209 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Bibliography

1. Zhai P., Chen X.B., and Schreyer D.J. (2013) Biofabrication. 5(1): 015009. 2. Selimoglu S.M., Ayyildiz-Tamis D., Gurhan I.D., and Elibol M. (2012) J Biosci Bioeng. 113(2):233-238.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Scientific context

Sodium alginate is used in biological experiments for the immobilization of cells and encapsulation due to its biocompatibility and simple gelation with divalent cations such as Ca2+. Studies suggest that preparation of alginate microspheres will sustain protein delivery within tissue scaffolds (1), as well as in many other cell types (2).

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Representative figure legend

Western Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 3G4-1F5 (SMC-209). Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody (SMC-209) at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa. Mouse Anti-Alginate Antibody [3G4-1F5] used in Western Blot (WB) on ALL BSA-Alginate Conjugate (SMC-209)