Swiss Prot
N/A
Subcellular locations
N/A
Gene ID
3791
Modification site
Tyr951
Applications
IF(IHC-P)
Concentration
1ug per 1ul
Excitation emission
590nm/617nm
Target Protein/Peptide
VEGFR2 Tyr951
Modification
Phosphorylation
Conjugated with
ALEXA FLUOR® 594
Conjugated
Alexa conjugate 1
Clonality
Polyclonal Antibody
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
Clone
Polyclonal Antibodies
Purification method
Purified by Protein A.
Group
Polyclonals and antibodies
Type
Conjugated Primary Antibody
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Host organism
Rabbit (Oryctolagus cuniculus)
Other name
Anti- VEGFR2(Tyr951) Polyclonal
Also known as
VEGFR2(Tyr951) Polyclonal Antibody
Properties
For facs or microscopy Alexa 1 conjugate.
Specificity
This antibody reacts specifically with VEGFR2(Tyr951)
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Antigen Source
KLH cunjugated synthetic phosphopeptide derived from human VEGFR2 around the phosphorylation site of Tyr951
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Synonyms
VEGFR2Tyr951; VEGFR2Y951; p-VEGFR2Tyr951; p-VEGFR2Y951; CD309; CD309 antigen; Fetal liver kinase 1; FLK-1; FLK1; KDR; Kinase insert domain receptor a type III receptor tyrosine kinase; Kinase insert domain receptor; KRD1; Ly73; Protein tyrosine kinase receptor FLK1; Protein-tyrosine kinase receptor flk-1; Tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; VEGFR 2; VEGFR; VEGFR-2; VEGFR2; VGFR2_HUMAN.
Background information
Hemangioblast MarkerVascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009].
